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Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation

机译：使用β-半乳糖苷酶互补检测法指导单链Fv进化为胞质表达可导致高度易受蛋白酶降解和聚集的蛋白质

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作者
Martineau Pierre; Philibert Pascal;
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年度 2004
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1. Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation [J] . Pascal Philibert, Pierre Martineau Microbial Cell Factories . 2004,第1期

机译：使用β-半乳糖苷酶互补检测法指导单链Fv进化为胞质表达可导致高度易受蛋白酶降解和聚集的蛋白质
2. Cloning and expression of functional single-chain Fv antibodies directed against NIa and coat proteins of potato virus Y. [J] . Rouis S, Lafaye P, Jaoua Aydi L, Journal of Virological Methods . 2006,第1期

机译：针对马铃薯N病毒的NIa和外壳蛋白的功能性单链Fv抗体的克隆和表达。
3. Cytoplasmic production of soluble and functional single-chain Fv-Fc fusion protein in Escherichia coli [J] . Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda Biochemical Engineering Journal . 2011,第3期

机译：大肠杆菌中可溶性和功能性单链Fv-Fc融合蛋白的细胞质产生
4. Direct observation of trap behaviors during degradation and breakdown evolution in highly stressed SiO/sub 2/ films by conductive atomic force microscopy [C] . Li Zhang, Milani, Y. Reliability Physics Symposium, 2005. Proceedings. 43rd Annual. 2005 IEEE International . 2005

机译：通过导电原子力显微镜直接观察高应力SiO / sub 2 /薄膜在降解和击穿演化过程中的陷阱行为
5. I. Development of a general method for assaying protein activity in vivo. II. Efforts toward the conversion of a penicillin-binding protein into a beta-lactamase by rational design and directed evolution. [D] . Sengupta, Debleena. 2004

机译：I.开发在体内测定蛋白质活性的通用方法。二。通过合理的设计和定向进化，努力将青霉素结合蛋白转化为β-内酰胺酶。
6. Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation [O] . Pascal Philibert, Pierre Martineau 2004

机译：使用β-半乳糖苷酶互补检测法指导单链Fv进化为胞质表达可导致高度易受蛋白酶降解和聚集的蛋白质
7. Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation [O] . Philibert, Pascal, Martineau, Pierre 2004

机译：使用β-半乳糖苷酶互补检测法指导单链Fv进化为胞质表达可导致高度易受蛋白酶降解和聚集的蛋白质
8. Protein Degradation in a TX-TL Cell-free Expression System Using ClpXP Protease. [R] . Sun, Z. Z., Kim, J., Singhal, V., 2014

机译：使用ClpXp蛋白酶在TX-TL无细胞表达系统中降解蛋白质。

Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation

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